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. 2012 Oct 23;21(1):8–17. doi: 10.1038/mt.2012.135

Figure 6.

Figure 6

SEM showing the fate of peridermal cells of the palatal MEE. Uninfected (a,b) wild-type and (c,d) Tgfβ3−/− mouse palates were examined by SEM at E14.5. (e–h) Tgfβ3−/− mouse embryos infected with Ad-GFP or Ad-GFP-TGFβ3 at E13.5 were harvested and examined at E18.5. Boxed areas in a,c,e, and g are shown in high magnification in b,d,f,h, respectively. At E14.5, the wild-type palatal MEE shows pebble-like appearance from the exposed basal cell layer (in b), whereas Tgfβ3−/− palatal MEE presents flat appearance with the intact peridermal cell layer (in d). Tgfβ3−/− mouse palatal shelves infected with Ad-GFP show persistent presence of the intact peridermal cell layer (in f), whereas Tgfβ3−/− palate rescued with Ad-GFP- TGFβ3 shows that peridermal cell layer of the MEE is disappeared, exposing the basal cell layer (in h). GFP, green fluorescent protein; KO, knockout; MEE, medial edge epithelium; SEM, scanning electron microscopy; TGFβ3, transforming growth factor β3; WT, wild-type.