Figure 1.
shRNA-B and shRNA-C knockdown Nav1.3 protein levels in HEK-Nav1.3 cells. (a) List of the short hairpin RNA (shRNA) target sequences used in this study against Nav1.3. (b) Western blot analysis using pan-sodium channel antibody on HEK-Nav1.3 cells lysates treated as indicated. β-Actin antibody was used to normalize the signal. (c) Graph depicting the quantification of the Nav1.3 protein in the different treatments. Note that shRNA-B and shRNA-C, but not shRNA-A or shRNA-Sc (scrambled control), significantly reduce Nav1.3 protein (P < 0.05). Data are presented as means ± SEM.