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. 2012 Aug 21;21(1):49–56. doi: 10.1038/mt.2012.169

Figure 6.

Figure 6

Nav1.3 knockdown in lumbar 4 (L4) dorsal root ganglion (DRG) neurons partially attenuates spared nerve injury (SNI)-induced mechanical allodynia. (a,b) Von Frey measurements of mechanical thresholds from hindpaws of SNI rats injected with (a) short hairpin RNA shRNA-Sc (n = 5) versus shRNA-B (n = 5), or (b) shRNA-Sc (n = 5) versus shRNA-C (n = 6) over the examined time course. SNI rats injected with AAV-GFP vector control (n = 5) are also shown (a,b). A partial but significant attenuation of mechanical allodynia over the indicated time course is observed when animals injected with AAV-GFP or shRNA-Sc are compared to those injected with (a) shRNA-B (P < 0.05), or (b) shRNA-C (P < 0.05). No difference was noted between animals injected with AAV-GFP versus animals injected with shRNA-Sc (P > 0.05). Animals injected with shRNA-B showed significant restoration of mechanical threshold at D10, D20, and D25 when compared to those injected with shRNA-Sc (*P < 0.05), or AAV-GFP (#P < 0.05). Animals injected with shRNA-C showed significant restoration of mechanical threshold at D20, and D25 when compared to those injected with shRNA-Sc (*P < 0.05), or AAV-GFP (#P < 0.05). AAV, adeno-associated virus; GFP, green fluorescent protein.