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. 2012 Sep 25;21(1):251–259. doi: 10.1038/mt.2012.202

Figure 2.

Figure 2

Prime-boost strategy enhances antigen-specific immune responses. Mice were immunized by 2 s.c. injections of 20 µg of naked or DOTAP gag with a 3-week interval. At week 8, some mice were boosted with 20 µg of the capsid protein p24 either subcutaneously (a and b) or intratracheally (c). T cell and B cell responses were analyzed 10 days after the p24 protein boost. (a) Number of Gag-specific interferon (IFN)-γ and interleukin-2 secreting T cells was determined by enzyme-linked immunosorbent spot (ELISPOT) on isolated spleen and lymph node cells. Means ± SEM are shown of a total of 5 mice per group. (b) Anti-p24 immunoglobulin (Ig)M, IgG1, and IgG2c titers were determined by serum enzyme-linked immunosorbent assay. Means ± SEM are shown for 5 mice, immunized with naked gag and 5 with DOTAP gag, each followed by p24 boost. (c) Number of Gag-specific IFN-γ secreting CD4 and CD8 T cells was determined by ELISPOT on isolated lung cells. Means ± SEM are shown of a total of 5 mice per group.