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. 2012 Sep 25;21(1):251–259. doi: 10.1038/mt.2012.202

Figure 4.

Figure 4

Type I IFN inhibitexpression of antigen-encoding mRNA and subsequent induction of immune responses. (a) Wild-type (WT) and interferon (IFN)αR−/− mice were immunized with 20µg DOTAP gag as previously described. Gag-specific IFN-γ and interleukin-2 secreting T cells were determined by enzyme-linked immunosorbent spot on isolated spleens. Mean of 5 mice per group is shown. ***P < 0.001. (b and c) WT, MyD88−/−, TRIF−/−, and IFNαR−/− bone marrow-derived DCs were transfected with 2.5 µg of DOTAP/DOPE-complexed Gag-encoding mRNA. Mean percentage ± SD of Gag+ CD11c+ cells (b) and expression pattern of the maturation markers major histocompatibility complex (MHC)-II, CD80, CD86, and CD40 on Gag+ and Gag- CD11c+ cells (c) was determined 24 hours later by flowcytometry. TRIF, TIR-domain-containing adapter-inducing interferon-β. (d) Flowcytometric analysis of DC subsets and inflammatory monocytes present in the draining lymph nodes of WT and IFNαR−/− mice 1 day post footpad injection of 4µg DOTAP gag. Conventional DCs were defined as CD11chi MHCIIint, tissue-derived DCs were CD11cint MHCIIhi and inflammatory monocytes were MHC-II+ Ly6chi CD11bhi. Mean ± SEM of 6 mice per group is shown.