Figure 2.

Canonical Wnt signaling is functional in MKs. Incubation of CHRF288-11 cells with Wnt3a results in a dose (A) and time (B; dose 150 ng/mL) dependent increase in cellular β-catenin levels as detected by Western blotting of whole cell lysates using an anti–β-catenin antibody. (C) Subcellular fractionation of cells treated with Wnt3a indicates that β-catenin accumulation occurs in subcellular fractions during a 24 hour period. (D) β-catenin accumulation in response to Wnt3a treatment is inhibited in a dose-dependent manner by the noncanonical agonist Wnt5a. (E) The canonical antagonist DKK1 inhibits β-catenin accumulation in a dose-dependent manner at an 8 hour time point. All blots are representative of at least 3 or more independent experiments.