Table 2. RFLP markers selected for screening the libraries and the BAC/BIBAC clones used as probes for FISH.
| ChromosomeNo. | SSR-LG | RFLP-LG | RFLP Marker | BAC Clone (No. Cells Examined for FISH Image) | BIBAC Clone (No. Cells Examined for FISH Image) |
|---|---|---|---|---|---|
| Ha01 | 1 | LG12 | 10D6 | 389J8 (64), 438D10 (12) | |
| Ha02 | 2 | LG14 | 8E4 | 62M10 (14) | |
| Ha03 | 3 | LG11 | 10C4 | 60O16 (26), 150N10a (21) | |
| 6E6 | 115B2 (22) | ||||
| Ha04 | 4 | LG13 | 15E3 | 67L19 (22) | 402M16 (6) |
| 11A6 | 380F19 (5), 407K6 (6) | ||||
| Ha05 | 5 | LG6 | 15D2 | 141K9 (29), 126N9 (10), 63A12b (21) | |
| Ha06 | 6 | LG4 | 14A2 | 61N8 (31) | |
| Ha07 | 7 | LG9 | 1C5 | 60L23 (15), 183P19 (14), 184P8 (9) | |
| Ha08 | 8 | LG7 | 7C1 | 115K11 (8) | |
| 21E6 | 429J21 (12) | ||||
| Ha09 | 9 | LG15 | 8C4b | 367P3a(9), 437F7 (19), 445H4 (18) | |
| 9D1 | 401C5 (5) | ||||
| LG18 | 13E4 | 84K7 (17) | |||
| Ha10 | 10 | LG16 | 8A1 | 78G18 (51) | |
| Ha11 | 11 | LG17 | 10B5a | 110G13 (10), 159N24 (23) | 368F18 (12), 428O6 (5) |
| Ha12t | 12 | LG5 | 2B4 | 481K13 (18) | |
| 2D4 | 155P12 (19) | ||||
| Ha13 | 13 | LG2 | 1E6 | 438A20 (8) | |
| 5E4 | 387P13 (18) | ||||
| Ha14t | 14 | LG1 | 20A5 | 374I4 (7), 386G6 (5) | |
| Ha15 | 15 | LG8 | 2E2 | 104I23 (7) | |
| 20F1 | 103H6 (27), 124A11 (9) | 470I10a (10) | |||
| Ha16 | 16 | LG3 | 9F2 | 59A24 (45) | |
| 4B6 | 464F20 (15) | ||||
| Ha17 | 17 | LG10 | 4D1 | 381J20a (16) | |
| 7D5 | 124J4 (12), 135J2b(12) | ||||
| Total | 27 | 24 (474) | 20 (270) |
Clone names in bold indicate the selected clones showing on the FISH map in Figure 2. BAC, bacterial artificial chromosome; BIBAC, bacterial /binary bacterial artificial chromosome; FISH, fluorescence in situ hybridization; LG, linkage group. RFLP, restriction fragment-length polymorphism; SSR, simple sequence repeats. t, numbering of the chromosomes is tentative.
a
Clones showing high background when used as a FISH probe with 100× blocking DNA; all the remaining clones were applied to 50× blocking DNA.
b
Clones containing pericentromeric heterochromatin sequences when used as a FISH probe.