Table 4. Spore viabilities, map distances, qualitative MMR phenotypes, and known mlh1 homolog phenotypes for the mlh3 alleles, msh5Δ, and mlh3 msh5 double mutants.
| Strain | Spore Viability, % | cM | MMR | mlh1 allele | MMR |
|---|---|---|---|---|---|
| mlh3 mutant analysis | |||||
| MLH3a | 97.0 | 100.9 (1068) | + | MLH1 | + |
| mlh3Δb | 71.7 | 54.5 (582) | − | mlh1Δ | − |
| mlh3-E31A | 89.2 | 67.0 (330) | − | mlh1-E31Ac,d | +/− |
| mlh3-N35A | 72.7 | 51.5 (229) | − | mlh1-E35A | ND |
| mlh3-A41F | 71.6 | 51.2 (214) | − | mlh1-A41F | ND |
| mlh3-G63R | 74.1 | 51.2 (216) | − | mlh1-G64R | ND |
| mlh3-K80E | 71.8 | 49.8 (221) | − | mlh1-K81Ee | − |
| mlh3-K83A | 94.1 | 100.5 (289) | + | mlh1-K84Ad | +/− |
| mlh3-R96A | 82.4 | 76.4 (177) | − | mlh1-R97Ad | − |
| mlh3-G97A | 81.5 | 61.0 (210) | − | mlh1-G98Ac,f | − |
| msh5 mutant analysis | |||||
| msh5Δa | 36.0 | 37.0 (540) | |||
| msh5Δ mlh3Δ | 31.8 | 38.5 (43) | |||
| msh5-D76Ag | 87.8 | 53.9 (77) | |||
| msh5-T423Ag | 95.2 | 78.3 (101) | |||
| msh5-D76A mlh3 R96A | 57.8 | 45.0 (81) | |||
| msh5-D76A mlh3 G97A | 47.1 | 31.7 (82) | |||
| msh5-T423A mlh3 R96A | 89.6 | 60.9 (160) | |||
| msh5-T423A mlh3 G97A | 78.3 | 54.7 (130) |
Spore viabilities (%) and cumulative genetic map distances from four spore-viable tetrads (number in parentheses) on chromosome XV are shown for wild-type, mlh3, and msh5 strains in the SK1 congenic EAY1108/1112 background (Table 2). The qualitative MMR phenotype of each allele (see Table 3) is shown for comparison. MMR data are also shown for the homologous mlh1 alleles, if known. MMR, mismatch repair; ND, not determined.
a
Data obtained from Argueso et al. (2004).
b
Data obtained from Nishant et al. (2008).
c
Data from Tran and Liskay (2000).
d
Data from Argueso et al. (2003).
e
Data from Wanat et al. (2007).
f
Data from Hoffman et al. (2003).
g
Data obtained from Nishant et al. (2010).