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. 2013 Jan 1;3(1):9–22. doi: 10.1534/g3.112.004622

Table 7. Genetic map distances for chromosomes III, VII, and VIII from single spores and tetrads with distributions of recombinant and parental progeny.

Single Spores
 Tetrads
Genotype n Par. Rec. cM n PD TT NPD cM
Chromosome III
 HIS4-LEU2
  Wild typea 2711 2360 351 12.9 572 413 141 2 12.6-15.0
  mlh3Δ 1453 1333 120 8.3 306 253 47 1 7.4-10.3
  mms4Δ 555 508 47 8.5 32 21 5 0 5.8-13.5
  mlh3Δ mms4Δ 1336 1304 32 2.4 170 158 2 0 0.2-1.1
 LEU2-CEN3
  Wild typea 2711 2527 184 6.8 572 488 68 0 5.4-6.8
  mlh3Δ 1453 1314 139 9.6 306 261 39 1 6.1-8.9
  mms4Δ 555 482 73 13.2 32 22 3 1 5.8-28.8
  mlh3Δ mms4Δ 1336 1302 34 2.5 170 156 4 0 0.6-1.9
 CEN3-MAT
  Wild typea 2711 2309 402 14.8 572 395 160 1 13.9-15.9
  mlh3Δ 1453 1246 207 14.2 306 223 78 0 11.7-14.2
  mms4Δ 555 464 91 16.4 32 23 3 0 2.6-8.9
  mlh3Δ mms4Δ 1336 1288 48 8.5 170 153 6 1 1.8-5.8
Chromosome VII
 TRP5-CYH2
  Wild typea 2711 1803 908 33.5 572 197 337 9 34.2-37.8
  mlh3Δ 1453 1215 238 16.4 306 198 100 0 15.4-18.2
  mms4Δ 555 391 164 29.5 32 11 11 0 19.7-30.3
  mlh3Δ mms4Δ 1336 1289 47 3.5 170 151 11 0 2.4-4.4
 CYH2-MET1:
  Wild typea 2711 2451 260 9.6 572 442 101 0 8.5-10.1
  mlh3Δ 1453 1350 103 7.1 306 266 32 0 4.5-6.3
  mms4Δ 555 500 55 9.9 32 18 4 0 5.0-13.2
  mlh3Δ mms4Δ 1336 1302 34 2.5 170 156 6 0 1.1-3.0
 MET13-LYS5:
  Wild typea 2711 2152 559 20.6 572 334 205 4 19.6-22.6
  mlh3Δ 1453 1307 146 10.0 306 242 55 1 8.7-11.7
  mms4Δ 555 461 94 16.9 32 15 7 0 10.9-20.9
  mlh3Δ mms4Δ 1336 1271 65 4.9 170 148 14 0 3.2-5.4
Chromosome VIII
 CEN8-THR1:
  Wild typea 2711 2105 606 22.4 572 317 219 2 20.2-22.8
  mlh3Δ 1453 1305 148 10.2 306 251 45 0 6.6-8.6
  mms4Δ 555 463 92 16.6 32 16 6 0 8.9-18.4
  mlh3Δ mms4Δ 1336 1288 48 3.6 170 157 3 0 0.4-1.5
 THR1-CUP1:
  Wild typea 2711 2043 668 24.6 572 277 260 1 23.5-25.9
  mlh3Δ 1453 1258 195 13.4 306 226 69 1 11.1-14.2
  mms4Δ 555 427 128 23.1 32 14 8 0 13.1-23.3
  mlh3Δ mms4Δ 1336 1292 44 3.3 170 154 6 0 1.1-2.6

Strains analyzed are isogenic derivatives of the SK1 NHY942/943 background (Tables 1 and 2). Single spore data are shown with n, total number of spores, and parental and recombinant data. Map distances (cM) were calculated by recombination frequency (recombinant spores/total spores) × 100. Tetrad data are shown with n, number of complete tetrads. Map distances (cM) were calculated using the Perkins formula (Perkins 1949), and 95% confidence intervals were calculated using the Stahl Laboratory Online Tools website (http://www.molbio.uoregon.edu/∼fstahl/).