Figure 1. Widespread Sox2-GFP expression in epithelial tissues of mice.

(A) Experimental outline for the generation and analysis of Sox2^GFP reporter mice. IHC, immunohistochemistry; FACS, fluorescence-activated cell sorting. (B) Sox2-GFP expression in targeted embryonic stem cells (ESCs), the inner cell mass of blastocysts (asterisk marks GFP-negative control blastocyst), and neonatal neural progenitor cells (NPCs). BF, brightfield. (C) IHC for GFP, Sox2 and the basal cell marker p63 on paraffin-embedded sections of adult testis, pylorus and corpus of the glandular stomach, esophagus, forestomach, and anus of wild type (Sox2^WT) and Sox2^GFP mice. Inserts also show cross-sections of corpus and pylorus. Asterisk marks unspecific antibody staining of blood vessels. Dashed lines outline gastric units (glandular stomach) or the basement membrane (testes, esophagus, forestomach, anus). Original magnifications as indicated. See also Suppl. Figure 1 and 2.