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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Stem Cells. 2013 Jan;31(1):23–34. doi: 10.1002/stem.1273

Figure 2.

Figure 2

Effective killing of glioblastoma stem cells by combination of 3-BrOP and BCNU. (A) Inhibition of GSC11 cells by BCNU (left panel), TMZ (right panel), or their combination with 3-BrOP under hypoxic conditions (2% O2 for 72 h, MTS assay). (B) Inhibition of GSC23 cells by BCNU (left panel), TMZ (right panel), or their combination with 3-BrOP under hypoxic conditions (2% O2 for 72 h, MTS assay). (C) Induction of cell death in GSC11 cells treated with BCNU, 3-BrOP, or their combination. Cells were incubated with the indicated concentrations of compounds for 24 h under normoxic and hypoxic conditions (2% O2). Cell viability was then measured using annexin-V/PI double staining followed by flow cytometry analysis. (D) Drug combination index (CI) calculated using the Calcusyn software in GSC11 cells treated with BCNU, 3-BrOP alone and in combination for 24 h under hypoxia (2% O2).