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Preferential killing of glioblastoma cells by 3-BrOP and BCNU. The cytotoxic effects of 3-BrOP, BCNU, or their combination was compared in (A) GSC23 cells, (B) serum (10% FBS)-induced GSC11 cells, (C) non-malignant human astrocytes (NHA), and (D) U87 glioma cells. Cells were incubated with the indicated drugs for 24 h under hypoxia (2% O2), and cell viability was measured by annexin-V/PI double staining followed by flow cytometry analysis.
