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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Stem Cells. 2013 Jan;31(1):23–34. doi: 10.1002/stem.1273

Figure 5.

Figure 5

Effect of 3-BrOP and BCNU or TMZ on energy metabolism in glioblastoma stem cells. (A) Inhibition of lactate production in GSC11 cells by the indicated concentrations of BCNU and 3-BrOP under hypoxia (2% O2). (B) ATP depletion in GSC11 cells by the indicated concentrations of BCNU and 3-BrOP for 3 h under hypoxia (2% O2). (C) ATP depletion in GSC11 cells by the indicated concentrations of BCNU and 3-BrOP for 6 h under hypoxia (2% O2). (D) ATP depletion in GSC23 cells by the indicated concentrations of BCNU and 3-BrOP for 3 h under hypoxia. (E) ATP depletion in GSC11 cells by the indicated concentrations of TMZ and 3-BrOP for 3 h under hypoxia (2% O2). (F) ATP depletion in GSC23 cells by the indicated concentrations of TMZ and 3-BrOP for 3 h under hypoxia (2% O2). (G) Viability of GSC11 cells after incubation with 3-BrOP and BCNU for 3 h or 6 h under hypoxic conditions (2% O2). Cell viability was measured by annexin-V/PI double staining followed by flow cytometry analysis. *, p<0.05; ***, p<0.001.