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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Stem Cells. 2013 Jan;31(1):23–34. doi: 10.1002/stem.1273

Figure 6.

Figure 6

Inhibition of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and hexokinase by BCNU and 3-BrOP. (A) Effect of BCNU (BC) and 3-BrOP (Br) on GAPDH enzyme activity in GSC11 cells. Cells were first incubated with the indicated concentrations of BCNU or 3-BrOP for 30 min, and protein extracts were prepared for analysis of GAPDH activity without further addition of the compounds in vitro. GAPDH activity was measured as described in Materials and Methods. (B) Inhibition of purified GAPDH enzyme by BCNU in vitro. (C) Inhibition of purified GAPDH enzyme by 3-BrOP in vitro. (D) Inhibition of purified GAPDH enzyme by the combination of BCNU and 3-BrOP in vitro. (E) Effect of BCNU, 3-BrOP, or their combination on hexokinase activity in GSC11 cells. Cells were first incubated with the indicated concentrations of compounds for 30 min, and protein extracts were prepared for analysis of hexokinase activity without further addition of the compounds in vitro. (F) Inhibition of purified hexokinase II enzyme by 3-BrOP in vitro.