TABLE 2.
Patient Demographics Grouped by Smoking Status
| Patient No. | Age (y) | Sex | Subsite | HPV-16 test result | T stage | N stage | M stage | Grade |
|---|---|---|---|---|---|---|---|---|
| Current smokers | ||||||||
| 1 | 56 | M | Tonsil | Positive | 1 | 0 | 0 | 4 |
| 2 | 73 | M | Tonsil | Negative | 4a | 2c | 0 | 3 |
| 3 | 54 | M | Base of tongue | Negative | 4 | 2b | 0 | 3 |
| Former smokers | ||||||||
| 4 | 48 | M | Tonsil | Positive | 4a | 2c | 0 | 3 |
| 5 | 64 | F | Tonsil | Positive | 1 | 2a | 0 | 4 |
| 6 | 66 | M | Base of tongue | Positive | 2 | 2b | 0 | 3 |
| Never smokers | ||||||||
| 7 | 46 | M | Tonsil | Positive | 3 | 2b | 1 | 3 |
| 8 | 49 | F | Tonsil | Positive | 2 | 0 | 0 | 4 |
| 9 | 73 | M | Tonsil | Negative | 2 | 2b | 0 | 3 |
Messenger RNA sequencing transcriptome data were analyzed for 9 patient-matched tumor and normal tissue samples. These patients were divided into analysis groups based on smoking status, including current smokers, former smokers with greater than 10 years of cessation, and never smokers. The human papillomavirus (HPV) status of each patient was determined by polymerase chain reaction (PCR) detection of HPV DNA and PCR quantification of E6 and E7 viral oncogenes. Additional clinical information collected was not used in grouping the patients for data analysis.