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. 2012 Dec 5;12:235. doi: 10.1186/1471-2148-12-235

Table 1.

Hypotheses for the main effects of several factors on larval time, pupal time, larval growth rate, pupal mass, chill-coma recovery time, and longevity

Traits
Factors
  Selection regime Inbreeding Rearing temperature Sex Adult temperature Feeding regime
Larval time
No
No
20°C > 27°C
Females > Males
-
-
Pupal time
No
No
20°C > 27°C
Males > Females
-
-
Growth rate
No
No
27°C > 20°C
Males > Females
-
-
Pupal mass
No
No
20°C > 27°C
Females > Males
-
-
Chill-coma recovery time
Controls > Selected Lines
No
27°C > 20°C
No
27°C > 20°C
No
Longevity No No 20°C > 27°C Females > Males 20°C > 27°C Control > Starvation

The hypotheses stated rest on prior results mainly obtained from B. anynana. Selection for increased cold tolerance is expected to have no effect on any trait other than chill-coma recovery time, as no correlated responses to selection were found in a previous study [34]. No inbreeding effects are expected here due to random mating for 14 generations after full-sib matings and thus prior to scoring trait values. Hypotheses regarding temperature, sex and feeding effects are all straight-forward and rest on previous results [e.g. [5,17,20,23,34,36,37]. ‘-‘: Not tested.