Table 3.
Results of nested ANOVAs for chill-coma recovery time
| A) Chill-coma recovery time | DF | MS | F | P |
|---|---|---|---|---|
| Selection Regime |
1,6 |
24368.4 |
22.0 |
0.0032 |
| Inbreeding Level |
2,6 |
1183.4 |
1.1 |
0.4005 |
| Replicate Line [Sel. & Inbr.] |
6,4738 |
1204.3 |
7.7 |
<0.0001 |
| Rearing Temperature |
1,4738 |
127708.0 |
815.0 |
<0.0001 |
| Adult Temperature |
1,4738 |
115643.0 |
738.0 |
<0.0001 |
| Sex |
1,4738 |
<0.1 |
<0.1 |
0.9926 |
| Selection Reg. * Inbreeding Level |
2,6 |
192.6 |
0.2 |
0.8448 |
| Selection Reg.* Rearing Temp. |
1,4738 |
480.4 |
3.1 |
0.0800 |
| Selection Reg. * Adult Temp. |
1,4738 |
1327.2 |
8.5 |
0.0036 |
| Selection Reg. * Sex |
1,4738 |
21.2 |
0.1 |
0.7131 |
| Inbreeding Level * Rearing Temp. |
2,4738 |
797.1 |
5.1 |
0.0062 |
| Inbreeding Level * Adult Temp. |
2,4738 |
692.4 |
4.4 |
0.0121 |
| Inbreeding Level * Sex |
2,4738 |
254.0 |
1.6 |
0.1979 |
| Rearing Temp. * Adult Temp. |
1,4738 |
1.2 |
<0.1 |
0.9298 |
| Rearing Temp. * Sex |
1,4738 |
1.8 |
<0.1 |
0.9140 |
| Adult Temp. * Sex |
1,4738 |
1384.2 |
8.8 |
0.0030 |
| Sel. Reg. * Inbreed. * Rear. Temp. |
2,4738 |
910.95 |
5.8 |
0.0030 |
| Sel. Reg. * Inbreed. * Adult Temp. |
2,4738 |
527.1 |
3.4 |
0.0347 |
| Inbreed. * Rear. Temp. * Adult Temp. |
2,4738 |
504.9 |
3.2 |
0.0400 |
| Rear. Temp. * Adult Temp. * Sex |
1,4738 |
1121.7 |
7.2 |
0.0075 |
| Error |
4738 |
156.7 |
|
|
|
B) Animals reared at 20°C |
DF |
MS |
F |
P |
| Selection Regime |
1,6 |
6782.9 |
18.8 |
0.0049 |
| Inbreeding Level |
2,6 |
213.8 |
0.6 |
0.5829 |
| Replicate Line [Sel. & Inbr.] |
6,1668 |
362.6 |
4.1 |
0.0004 |
| Adult Temperature |
1,1668 |
44483.1 |
501.1 |
<0.0001 |
| Sex |
1,1668 |
1.3 |
<0.1 |
0.9046 |
| Selection Reg. * Adult Temp. |
1,1668 |
1458.2 |
16.4 |
<0.0001 |
| Error |
1668 |
88.8 |
|
|
|
C) Animals reared at 27°C |
DF |
MS |
F |
P |
| Selection Regime |
1,6 |
23033.8 |
15.3 |
0.0079 |
| Inbreeding Level |
2,6 |
2340.1 |
1.5 |
0.2859 |
| Replicate Line [Sel. & Inbreed.] |
6,3059 |
1506.7 |
7.9 |
<0.0001 |
| Adult Temperature |
1,3059 |
81814.6 |
427.7 |
<0.0001 |
| Adult Feeding Regime |
1,3059 |
5.6 |
<0.1 |
0.8644 |
| Sex |
1,3059 |
0.4 |
<0.1 |
0.9620 |
| Selection Reg. * Inbreeding Level |
2,6 |
959.2 |
0.6 |
0.5611 |
| Selection Reg. * Adult Temp. |
1,3059 |
321.2 |
1.7 |
0.1952 |
| Selection Reg. * Adult Feed. |
1,3059 |
66.0 |
0.3 |
0.5570 |
| Selection Reg. * Sex |
1,3059 |
38.0 |
0.2 |
0.6557 |
| Inbreeding Level * Adult Temp. |
2,305 |
1643.0 |
8.6 |
0.0002 |
| Inbreeding Level * Adult Feed. |
2,3059 |
211.2 |
1.1 |
0.3317 |
| Inbreeding Level * Sex |
2,3059 |
145.2 |
0.8 |
0.4682 |
| Adult Temp. * Adult Feed. |
1,3059 |
231.8 |
1.2 |
0.2711 |
| Adult Temp. * Sex |
1,3059 |
3459.7 |
18.1 |
<0.0001 |
| Adult Feed. * Sex |
1,3059 |
174.4 |
0.9 |
0.3397 |
| Sel. Reg. * Inbreed. * Adult Temp. |
2,3059 |
961.3 |
5.0 |
0.0066 |
| Inbreed. * Adult Feed. * Sex |
2,3059 |
1243.4 |
6.5 |
0.0015 |
| Adult Temp. * Adult Feed. * Sex |
1,3059 |
1362.7 |
7.1 |
0.0076 |
| Error | 3059 | 191.3 |
Results for the effects of selection regime (selection vs. control), inbreeding level (C, I1, I2), replicate line, rearing temperature (20 vs. 27°C), adult temperature (20 vs. 27°C), and sex on chill-coma recovery time in Bicyclus anynana (A). In Tables B (animals reared at 20°C) and C (animals reared at 27°C) data were analyzed separately for each rearing temperature, as different feeding treatments were employed in animals reared at 27°C only. Consequently, the factor rearing temperature was dropped from both analyses, and the factor adult feeding regime (control vs. starvation) was added to Table C. For details see Material and Methods. Replicate line (random factor) was nested within selection regime and inbreeding level throughout. Minimum adequate models were constructed by sequentially removing non-significant interaction terms. Significant P-values are given in bold.