Table 4.
Results of nested ANOVAs for longevity
| A) Longevity | DF | MS | F | P |
|---|---|---|---|---|
| Selection Regime |
1,6 |
234.0 |
0.6 |
0.4616 |
| Replicate Line [Sel. & Inbr.] |
6,3744 |
404.9 |
2.8 |
0.0109 |
| Inbreeding Level |
2,6 |
146.3 |
0.4 |
0.6967 |
| Rearing Temperature |
1,3744 |
264262.0 |
1806.6 |
<0.0001 |
| Sex |
1,3744 |
20.7 |
0.14 |
0.7070 |
| Selection Reg. * Inbreeding Level |
2,6 |
2020.7 |
5.3 |
0.0440 |
| Selection Reg. * Rearing Temp. |
1,3744 |
93.4 |
0.6 |
0.4242 |
| Selection Reg. * Sex |
1,3744 |
52.2 |
0.4 |
0.5505 |
| Inbreeding Level * Rearing Temp. |
2,3744 |
440.1 |
3.0 |
0.0495 |
| Inbreeding Level * Sex |
2,3744 |
153.7 |
1.0 |
0.3498 |
| Rearing Temp. * Sex |
1,3744 |
5324.0 |
36.4 |
<0.0001 |
| Sel. Reg. * Inbreed. * Rear. Temp. |
2,3744 |
472.0 |
3.2 |
0.0398 |
| Sel. Reg. * Inbreed. * Sex |
2,3744 |
940.0 |
6.4 |
0.0016 |
| Sel. Reg. * Rearing Temp. * Sex |
1,3744 |
1.5 |
<0.1 |
0.9202 |
| Inbreed. * Rear. Temp. * Sex |
2,3744 |
178.4 |
1.2 |
0.2954 |
| Four-way Interaction |
2,3744 |
932.1 |
6.4 |
0.0017 |
| Error |
3744 |
146.3 |
|
|
|
B) Animals reared at 20°C |
DF |
MS |
F |
P |
| Selection Regime |
1,6 |
287.5 |
0.3 |
0.6267 |
| Inbreeding Level |
2,6 |
174.0 |
0.2 |
0.8570 |
| Replicate Line [Sel. Reg. & Inbr.] |
6,1312 |
1106.3 |
4.8 |
<0.0001 |
| Adult Temperature |
1,1312 |
34430.6 |
148.6 |
<0.0001 |
| Sex |
1,1312 |
2568.9 |
11.1 |
0.0009 |
| Selection Reg. * Inbreeding Level |
2.6 |
1712.1 |
1.6 |
0.2864 |
| Selection Reg. * Adult Temp. |
1,1312 |
1726.0 |
7.5 |
0.0064 |
| Selection Reg. * Sex |
1,1312 |
7.3 |
<0.1 |
0.8589 |
| Inbreeding Level * Adult Temp. |
2,1312 |
3034.3 |
13.5 |
<0.0001 |
| Inbreeding Level * Sex |
2,1312 |
131.9 |
0.6 |
0.5659 |
| Adult Temperature * Sex |
1,1312 |
2762.2 |
12.6 |
0.0004 |
| Sel. Reg. * Inbreed. * Sex |
2,1312 |
1395.7 |
6.0 |
0.0025 |
| Error |
1312 |
231.6 |
|
|
|
C) Animals reared at 27°C |
DF |
MS |
F |
P |
| Selection Regime |
1,6 |
38.0 |
0.1 |
0.7603 |
| Inbreeding Level |
2,6 |
142.8 |
0.4 |
0.6974 |
| Replicate Line [Sel. Reg. & Inbr.] |
6,2394 |
37. |
7.0 |
<0.0001 |
| Adult Temperature |
1,2394 |
1177.7 |
21.8 |
<0.0001 |
| Adult Feeding Regime |
1,2394 |
52827.7 |
977.2 |
<0.0001 |
| Sex |
1,2394 |
3684.1 |
68.1 |
<0.0001 |
| Selection Reg. * Inbreeding Level |
2,6 |
475.6 |
1.3 |
0.3451 |
| Selection Reg. * Adult Temp. |
1,2394 |
104.6 |
1.9 |
0.1644 |
| Selection Reg. * Adult Feed. |
1,2394 |
49.3 |
0.9 |
0.3399 |
| Selection Reg. * Sex |
1,2394 |
15.4 |
0.3 |
0.5936 |
| Inbreeding Level * Adult Temp. |
2,2394 |
37.1 |
0.7 |
0.5033 |
| Inbreeding Level * Adult Feed. |
2,2394 |
112.8 |
2.1 |
0.1244 |
| Inbreeding Level * Sex |
2,2394 |
137.9 |
2.6 |
0.0782 |
| Adult Temp. * Adult Feed. |
1,2394 |
451.8 |
8.4 |
0.0039 |
| Adult Temp. * Sex |
1,2394 |
114.8 |
2.1 |
0.1451 |
| Adult Feed. * Sex |
1,2394 |
1481.2 |
27.4 |
<0.0001 |
| Sel. Reg. * Inbreed. * Adult Temp. |
2,2394 |
17.4 |
0.3 |
0.7248 |
| Sel. Reg. * Inbreed. *Adult Feed. |
2,2394 |
532.5 |
9.9 |
<0.0001 |
| Sel. Reg. * Inbreed. * Sex |
22394 |
0.7 |
<0.1 |
0.9879 |
| Sel. Reg. * Adult Temp. * Sex |
2,2394 |
1.7 |
<0.1 |
0.8610 |
| Sel. Reg. * Adult Temp. * AdFeed. |
1,2394 |
128.8 |
2.4 |
0.1229 |
| Sel. Reg. * Adult Feed. * Sex |
1,2394 |
0.7 |
<0.1 |
0.9110 |
| Inbreed. * Adult Temp. * AdFeed. |
2,2394 |
14.4 |
0.3 |
0.7659 |
| Inbreed. * Adult Temp. * Sex |
2,2394 |
161.9 |
3.0 |
0.0502 |
| Inbreed. * Adult Feed. * Sex |
2,2394 |
164.2 |
3.0 |
0.0482 |
| Adult Temp. * Adult Feed. * Sex |
1,2394 |
87.1 |
1.6 |
0.2044 |
| Inbreed. * AdT * AdF * Sex |
2,2394 |
222.8 |
4.1 |
0.0163 |
| Error | 2394 | 54.0 |
Results for the effects of selection regime (selection vs. control), inbreeding level (C, I1, I2), replicate line, replicate cage, rearing temperature (20 vs. 27°C), and sex on longevity in Bicyclus anynana (A). In Tables B (animals reared at 20°C) and C (animals reared at 27°C) data were analyzed separately for each rearing temperature, as different feeding treatments were employed in animals reared at 27°C only, and as different adult temperatures were employed in animals reared at 27°C only temporarily. Consequently, the analysis in A was restricted to the animals fed ad libitum and kept as adults at 27°C, to mainly explore effects of rearing temperature. The factor rearing temperature was replaced by the factor adult temperature in analyses B and C, and the factor adult feeding regime (control vs. starvation) was added to Table C. For details see Material and Methods. Replicate line (random factor) was nested within selection regime and inbreeding level throughout. Minimum adequate models were constructed by sequentially removing non-significant interaction terms. Significant P-values are given in bold.