Table 2. DNA markers used for fine mapping spring1 on chromosome 7.
| Marker | BAC | Mt3.5 Genome assembly | UMN (cM) | Type | Forward Primer | Reverse Primer | cBand Size (bp) | dLate F2 | dEarly F2 | dLate Test Cross | dEarly Test Cross |
| ah2_9|7d | CT967302 | chr7:17913549..17913763 | 42.4 | SSR | AAGGGCCACAAAGAGAAGGT | GGTGGAATCAAAGCACCAAT | 215 | 7/57 | NT | NT | NT |
| ah2_172c4a | AC146784 | chr7:19580059..19580346 | 47.0 | SSR | GCCGAATATGCGAGCTTTT | TGGCTCACCTTCCACTTCA | 288 | 6/57 | NT | NT | NT |
| a003B12 | AC159114 | chr7:21245026..21245168 | 47.7 | SSR | GCTTTTGAGGTCAAAGTACAA | CAGGGAGTAGCTTGTAAATCA | 143 | 4/57 | NT | NT | NT |
| ah4_42i13a | AC161750 | chr7:21396080..21396350 | 47.7 | SSR | TGTATGCCAAGCATCGGTTA | TGCCAAGAGGAAACTTGGTT | 271 | 4/57 | NT | NT | NT |
| aH2_142N19_fr1 | AC147179 | chr7:22856921..22857163 | 50.4 | SSR | CCAATTTTGACACCCTACAT | GAGATTAAATCCACCCCTTC | 243 | 1/57 | NT | NT | NT |
| Medtr7g080490 | AC147179 | chr7:22879847..22880323 | – | indel | GCCAGCTCCAGCTGACATTGTGG | CCCTTGAGGTCAGTCTCAACCGT | 477 | 1/57 | 3/419 | 1/95 | 3/83 |
| ah2_14c17b | AC142095 | chr7:23179073..23179192 | 50.4 | SSR | AGCTGCTCTCAGTGCCATTT | TGCGTGTGACAAGTTCCTCT | 120 | 0/57 | NT | 1/95 | 2/83 |
| a003A09 | AC133780 | chr7:24672851..24672966 | 50.4 | SSR | TTGTGGTGACTAGTGATTGG | ATGTGAAGTAAATCCCTTGC | 116 | 0/57 | 3/419 | 0/95 | 0/83 |
| MtCO | AC133780 | chr7:24688717..24688738 | – | indel | GAATAGAGGGGATGCCATGTT | CAAATCGCCCTCTCACTCTC | 381 | NT | 3/419 | NT | NT |
| TF_B3 | AC149131 | chr7:24783230..24783575 | – | indel | TCCGACTGTTATTGTTAGGCCA | TGCAGAAAGCAGCAGAGGCT | 346 | NT | 2/419 | NT | NT |
| eIF-4gamma | AC149131 | chr7:24840562..24840940 | – | indel | TCTGGCTATTGTGAGATGGGTCT | ACGGTTGTATGTTTTGCTTACTGC | 379 | NT | 1/419 | NT | NT |
| Medtr7g084090.1_SUVH4 | AC155894 | chr7:24973864..24974348 | – | indel | TCCAGGACGCTATCAAGCGCA | CGAGTGGTGCTGTTTGCCGC | 485 | NT | 1/419 | NT | NT |
| Medtr7g084170.1 | AC186135 | chr7:25016694..25017139 | – | indel | AGGAAATTCTACCAAAGATTCTGAG | TCATCCAAGTTTGACCGTTTTCT | 446 | NT | 1/419 | NT | NT |
| Medtr7g084560.1 | AC157890 | chr7:25212756..25213142 | – | indel | AGCATTGACAGGATTTCGTGATGC | TGGCCACTACGATCCAGCTCA | 387 | NT | 0/419 | NT | NT |
| FTa1 | AC123593 | chr7:25433850..25434142 | – | indel | CCTTCAAAATATGAAAAAGGGCTA | AAATTTAAAATGTCTTCCTTGCTC | 293 | 0/57 | 0/419 | 0/95 | 0/83 |
| Medtr7g085120 | AC123593 | chr7:25499863..25500262 | – | indel | GCTGGTAGCACTGGTAGCCGTA | GCAGCTTGTTCCGGATCATTTGCAT | 400 | 0/57 | NT | 0/95 | 0/83 |
| Medtr7g085190.1 | AC145753 | chr7:25527032..25527377 | – | indel | TGAGGCTTATTGTTCAAGTCAAGGA | TGGTAAACCTTATGCTGAGAGGA | 346 | 1/57 | 0/419 | 0/95 | 0/83 |
| Medtr7g085200 | AC145753 | chr7:25537421..25538020 | – | indel | CCCAAGTTTCCCAAAACATAGT | GGTTTTGGTTTCATGAGAGATGGT | 600 | 1/57 | NT | 0/95 | 4/83 |
| AC145753_RR1 | AC145753 | chr7:25593027..25593223 | – | SSR | ACGAGGAACGGTGGCAGAAGGA | TGGTTGGTCTTGTAATATTGGC | 197 | 2/57 | NT | NT | NT |
| AC167711_RR8 | AC167711 | chr7:25677751..25677868 | – | SSR | TGCTGAGGCACGTCCCAGTG | ACCCCTGAAACCAGACAAGCCA | 118 | 3/57 | NT | NT | NT |
| AC167711_RR5 | AC167711 | chr7:25695231..25695594 | – | SSR | GTGAAGAGCAAGGGGTCGCGT | TGATACAACAAAGACAAACCACAGGCT | 364 | 3/57 | NT | NT | NT |
| AC148816_RR1 | AC148816 | chr7:25943053..25943342 | – | SSR | TCAGCTTGGGAACGTTGGTCGT | GGAGGCGCATCATCACCCCG | 290 | 4/57 | NT | NT | NT |
| AC148816_RR4 | AC148816 | chr7:25952355..25952590 | – | SSR | GGGTCATGGCCTTGGACCACA | ACCTCCTCCAACAAAAGCCACCT | 236 | 4/57 | NT | NT | NT |
| AC148816_RR5 | AC148816 | chr7:25957056..25957337 | – | SSR | AGACGATGATGAAGGTGAGGATGGA | TCAGCCTTCTTTTCCAAGAGTGCTTC | 282 | 4/57 | NT | NT | NT |
| AC148816_RR12 | AC148816 | chr7:26039826..26040091 | – | SSR | TGGCTGAATAACTGTTGTGCAAGGCT | TCAGTGGACGATCGTTGATACTTGTG | 266 | 4/57 | NT | NT | NT |
| a002E05 | AC126009 | chr7:26164550..26164669 | 53.3 | SSR | ATGGAAGGTGGAACCTATCT | GGTGTCGACTGATCCTAGC | 120 | 6/57 | NT | NT | NT |
| a005G08 | AC141107 | chr7:27227337..27227598 | 53.3 | SSR | GGTTTACTGGCCCTCAACAA | CTCCGTATGCCTTTCTTCCA | 262 | 13/57 | NT | NT | NT |
| ah2_81g19a | AC153128 | chr7:28185897..28186094 | 56.1 | SSR | GTTCCAAAAACGCACCAAGT | CATGACAGCAGTACATTGCC | 198 | 20/57 | NT | NT | NT |
| bMTIC714 | AC126016 | chr7:34518760..34518883 | 57.0 | SSR | TAGAAAAGCACAACAAGCTG | TGCTACGTATCAAATCAACAA | 124 | 29/57 | NT | NT | NT |
DNA markers from the MtCO region of chromosome 7 were analysed for linkage to spring1 in the mapping crosses (see Text). The interval containing spring1 is flanked by Medtr7g084170.1 and Medtr7g085190.1 shown in bold; each marker is separated from spring1 by one recombination event.
DNA marker taken from the University of Minnesota (UMN) Integrated Genetic Map of Medicago truncatula http://www.medicago.org/genome/map.php.
DNA marker from Pierre et al (2010).
Size of PCR fragment predicted from Mt3.5 Genome assembly from http://medicagohapmap.org/.
Numbers of recombinants detected by the marker in the late or early flowering classes from the F2 of the “spring1 x Jester” cross, or from the Test cross (see Table 1 and Text). “Test Cross” is (♂(♂spring1 x ♀Jester) x ♀Jester). NT is not tested. Indel is insertion/deletion; SSR is Simple Sequence Repeat. The column entitled Mt3.5 Genome assembly gives the position of the marker on the pseudomolecule from Mt3.5 Genome assembly from http://medicagohapmap.org/after a Chromosome Visualisation Tool (CViT) BLAST search http://medicagohapmap.org/was carried out.