Figure 1. Vitellogenin purification using ion exchange chromatography.

Con A-purified vitellogenin [31] was subjected to ion exchange chromatography in a MonoQ column (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA). The absorbance of the eluate was continuously recorded at 280 nm (continuous line). The dotted line shows the discontinuous NaCl gradient. The inset at the left shows the Coomassie Blue R stained SDS-PAGE of the pooled fractions (arrow above the A_280nm profile).