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. 2013 Jan 7;8(1):e53460. doi: 10.1371/journal.pone.0053460

Figure 2. Concanavalin A (Con A) interaction with the purified vitellogenin polypeptides.

Figure 2

Purified vitellins of O. tipulae were subjected to SDS-PAGE (T = 10%) and transferred to a nitrocellulose membrane (Hybond-C, Life Technologies). All lanes, except lane (b) with 0.5 µg of protein, contained 5 µg of purified O. tipulae vitellogenin. Each lane of the membrane was cut and incubated separately in the presence (b, c, d and f) or absence (e) of Concanavalin A (50 µg/ml). (a) Coomassie Blue-stained SDS-PAGE of purified vitellins. (b and c) Membrane strips incubated with horseradish peroxidase (50 µg/mL). (d) Membrane strip incubated in the absence of horseradish peroxidase. (f) Membrane strip incubated in the presence of horseradish peroxidase (50 µg/mL) and 0.2 M α-methyl mannoside.