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. 2013 Jan 7;8(1):e53546. doi: 10.1371/journal.pone.0053546

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© 2013 Culver et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Coronal brain sections were imaged, and regions of interest (ROIs) where Hypoxyprobe-1 (hpi) staining was observed were selected for closer inspection. (B–J) We observed colocalization between Hypoxyprobe-1 and Hif1α in each of the three ROIs shown, and in cells located in the SEZ, striatum, and ependymal layer. Overall, we observed colocalization with Hif1α in 83% of the cells that exhibited bright Hypoxyprobe-1 staining (n_cells = 18). Image data were processed with a Gaussian filter. Representative images are shown.