Figure 2. EphA2^−/− mice have reduced post-stroke blood-brain barrier (BBB) leakage, edema and infiltrating immune cells.

(A) Quantitative measurement of brain edema in ipsilateral brain hemisphere by the wet-dry method. EphA2^−/− mice have lower levels of post-stroke brain edema in comparison with wild type mice. (B) Quantitative measurement of Evans Blue dye extravasation in ipsilateral brain hemisphere. Evans Blue dye content was significantly lower in brains from EphA2^−/− mice compared with wild type. (C) Flow cytometry analysis of the ipsilateral hemisphere following 72 h of reperfusion showed significantly fewer infiltrating (CD45^high) immune cells in EphA2^−/− mice compared to vehicle treated controls. Data are means ± SEM from 12 to 13 animals. **p<0.01 relative to wild type.