Table 5. Symbiotic properties of partially effective and ineffective mutants.
Inoculum strain | Genotype | Mean wet foliage weight in mga | % effectiveness based on wet weight | Acetylene reductionb |
none | 19.1±3.0** | 22.2 | 0 | |
R7A | Wild-type | 86.0±32.2 | 100 | 100±35 |
JS24 | msi158::lacZ | 59.2±9.8* | 68.8 | 51.5±18.2* |
JS27 | Δ [msi262-263]:: Ωkan | 64.2±22.7 | 74.6 | 72.6±33.5 |
JS42 | nifQ::lacZ | 61.9±12** | 71.9 | 64.0±15.4* |
JS28 | Δ [fdxN-fixU]:: Ωkan | 17.46±11.6** | 20.3 | 0 |
JS29 | Δ [nifZ-fixU]:: Ωkan | 23.6±8.3** | 27.4 | 0 |
JS30 | ΔfixU:: Ωkan | 67.5±21.7 | 78.4 | 103±22.2 |
Mean wet foliage weight of 30 plants ± Standard Deviation. Data were analysed using the Students T-test. b Percentage of acetylene reduction relative to R7A. Nitrogen fixation activity was measured as the amount of C2H2 reduced (µmol h−1) per plant root for 10 plants ± standard deviation. Data were analysed using the Students T-test. A single asterisk represents P<0.05 and two asterisks P<0.005 when compared to R7A.