Table 3. Antigens downregulated in metastasis.
Antigen | Cell line (% Positivity) | ||
SW480 | SW620 | SW480/SW620 ratio | |
EGFR | 51.50 | 1.12 | −45.98 |
CD21 | 22.84 | 0.51 | −44.78 |
CD56/NCAM | 49.48 | 1.17 | −42.29 |
CD184/CXCR4 | 17.55 | 0.62 | −28.31 |
CD73 | 22.64 | 0.88 | −25.73 |
CD120b | 35.33 | 1.65 | −21.41 |
CD57 | 94.71 | 5.10 | −18.57 |
CD162/SELPLG | 8.49 | 0.51 | −16.65 |
CD7 | 6.31 | 0.51 | −12.37 |
CD70 * | 7.19 | 0.59 | −12.19 |
CD87/uPAR | 7.33 | 0.63 | −11.63 |
CD3 * | 5.79 | 0.70 | −8.27 |
CD95/FASR | 62.95 | 7.84 | −8.03 |
CD8b * | 6.55 | 0.82 | −7.98 |
CD33 * | 5.76 | 0.77 | −7.48 |
CDw93 * | 5.14 | 0.72 | −7.14 |
CD209/DC-SIGN | 10.76 | 2.16 | −4.98 |
CD45RA * | 5.83 | 1.22 | −4.78 |
CD91/LRP-1 | 13.88 | 2.94 | −4.72 |
CD79b * | 7.32 | 1.62 | −4.52 |
CD153 * | 11.70 | 2.65 | −4.42 |
CD130/gp130 | 9.15 | 2.16 | −4.24 |
CD337/NCR3 * | 7.32 | 1.74 | −4.21 |
CD100/SEMA4D | 6.60 | 1.58 | −4.18 |
CD193/CCR3 * | 8.01 | 1.97 | −4.07 |
CD271/LNGFR | 76.08 | 19.47 | −3.91 |
CD181/IL8RA * | 5.07 | 1.31 | −3.87 |
CD6 * | 8.09 | 2.10 | −3.85 |
CD243/P-gp * | 52.5 | 13.82 | −3.80 |
CD61/Integrin β3 * | 5.22 | 1.51 | −3.46 |
CD75 * | 8.54 | 2.55 | −3.35 |
CD107b * | 7.61 | 2.32 | −3.28 |
CD108 | 7.16 | 3.22 | −2.22 |
CD54/ICAM1 | 57.18 | 27.45 | −2.08 |
CD55 | 73.75 | 36.77 | −2.01 |
Antibody array results showing surface antigens that were at least two-fold decreased in cell positivity in SW620 (metastatic) as compared to SW480 (primary). Abbreviations: epidermal growth factor receptor, EGFR; neural cell adhesion molecule, NCAM; C-X-C chemokine receptor 4, CXCR4; selectin P ligand, SELPLG; urokinase receptor, UPAR; FAS receptor, FasR; dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin, DC-SIGN; low density lipoprotein receptor related protein 1, LRP1; glycoprotein 130, gp130; natural cytotoxicity triggering receptor 3, NCR3; semaphorin-4D, SEMA4D; C-C chemokine receptor 3, CCR3; low affinity nerve growth factor receptor; LNGFR; interleukin 8 receptor alpha, IL8RA; P-glycoprotein, P-gp; intercellular adhesion molecule 1, ICAM1.
, Antigen was not two-fold decreased by comparison of mean fluorescence intensities (Table S4).