Figure 7. tgs1 deletion compromised the ability of the Mtb to go into a dormant state.

(A) Auramine-O and Nile red staining of WT and Δ-tgs1 mutant and Δ-tgs1 complemented strain (Δ-tgs1C+) population in in vitro granuloma. (B) Alamar Blue readouts at different time intervals when WT and Δ-tgs1 mutant and Δ-tgs1 complemented strain from granulomas treated with anti-TNF-α antibody or IgG, were incubated with Alamar blue dye. (data of one representative data set has been displayed.) (C) Δ-tgs1 mutant from granulomas treated with IgG or anti-TNF-α antibody had a lower antibiotic resistance as compared to WT Mtb cells from IgG treated granulomas. Data are presented as mean +/− SEM from 3 experiments. P values were calculated using students t test. *p<0.05.