Table 3.

Genetically modified organisms constructed in the present study: Mutant strains of C. glutamicum based on the wild type strain (ATCC 13032) were constructed by site-directed mutagenesis

Strain	Genotype	Reference
C. glutamicum ATCC 13032	wild type	American Type Culture Collection
C. glutamicum Δpgi	deletion of the pgi gene (NCgl0817)	This work
C. glutamicum ΔpurA	deletion of the purA gene (NCgl2669)	This work
C. glutamicum ΔguaB2	deletion of the guaB2 gene (NCgl0578)	This work
C. glutamicum purFK348Q	Nucleotide exchange K348Q in the purF gene (NCgl2495)	This work
C. glutamicum ΔpurA ΔguaB2	double deletion of purA and guaB2 genes	This work
C. glutamicum ΔpurA ΔguaB2 purF348Δpgi	deletion of purA, guaB2 and pgi genes and allelic amino acid substitution K348Q in the purF gene	This work
E. coli DH5α	F−endA1, hsdR17(rk−mk+) supE44, thi-1λ− recAI gyrA96 relA1 Φ80ΔlacAm15	[48]
E. coli NM522pTC	supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK−mK+) [F′ proAB lacIqZΔM15]	Stratagene

E. coli strains were employed for plasmid construction.