Table 4.
Intracellular and extracellular pH (pHi/ e) and acid–base parameters in white muscle/ liver tissue homogenates and blood, respectively, from N. rossii. Control: 1°C, 0.04 kPa CO2; warm normocapnia: 7°C, 0.04 kPa CO2; cold hypercapnia: 1°C, 0.2 kPa CO2; warm hypercapnia: 7°C, 0.2 kPa CO2
|
Acclimation |
White muscle homogenate |
Liver homogenate |
Blood acid–base parameters |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T (°C) | PCO2 (kPa) | N | PCO2 (kPa) | [HCO3-] (mmol*l-1) | pHi | N | PCO2 (kPa) | [HCO3-](mmol*l-1) | pHi | N | PCO2 (kPa) | [HCO3-] (mmol*l-1) | pHe |
| 1 |
0.04 |
3 |
0.640±0.126 |
3.994±0.410 |
7.325±0.065 |
3 |
2.555±0.270 |
9.213±0.379 |
7.080±0.027 |
9 |
1.18±0.19 |
8.05±0.56 |
7.438±0.055 |
| 7 |
0.04 |
5 |
1.071±0.123 |
5.294±0.335 |
7.242±0.037 |
1 |
3.616 |
8.020 |
6.890 |
9 |
1.16±0.05 |
6.31±0.28a,b |
7.315±0.045b |
| 1 |
0.2 |
5 |
0.936±0.117 |
6.724±0.664a |
7.383±0.027 |
5 |
3.031±0.484 |
10.107±1.197 |
7.053±0.066 |
5 |
1.46±0.09 |
11.28±0.32a,b,c |
7.508±0.028 |
| 7 | 0.2 | 10 | 1.296±0.167 | 6.849±0.373a | 7.290±0.038 | 7 | 4.495±0.521 | 10.347±0.724 | 6.920±0.039 | 10 | 1.41±0.11 | 10.08±0.57a | 7.507±0.037 |
Data are presented as means ± SEM. Superscript letters indicate following significant differences: a – sign. different to control conditions, b – sign. different to temperature & CO2 acclimation. c –sign. different to temperature acclimation (t-test, ANOVA, p≤0.05).