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. 2012 Dec 7;13:66. doi: 10.1186/1471-2172-13-66

Figure 3.

Figure 3

The gating strategy used to identify the different immune cell populations. Isolated cells from blood and endocervix were first gated based on forward scatter and side scatter. T cells were then identified based on CD3 positivity and NK cells were identified based on CD3-CD56+ profile. From the T cell CD3+ gate, CD4+ and CD8+ T cells were delineated. Following immune cell identification, perforin and granzyme expression were assessed.