Figure 3. sCD14 level in the plasma of CAR-primed endotoxin shock-induced model mice at various doses of LPS.

Plasma was collected from CAR-primed C57BL/6 mice that were administered with LPS at the indicated time. Soluble CD14 in the plasma was detected as follows: Each plasma sample was diluted 20 folds with saline, and then subjected to SDS-PAGE and subsequent western blotting. Then, sCD14 on the membrane was detected using anti-mouse CD14 polyclonal antibody.