Figure 5. Histological examination of CAR- or d-galN-primed endotoxin shock-induced mice liver.

(A) LPS (20 μg/mouse) was administered i.v. to CAR (5 mg/mouse)-primed C57BL/6 mice, and 12 h later, the livers were extirpated from these mice. (B) LPS (0.5 μg/mouse) was administered i.v. to D-galN (20 mg/mouse)-primed C57BL/6 mice. The livers were extirpated after 6 h of LPS injection. (C) Untreated mice were used as negative control. The extirpated livers were fixed in 10%-formaldehyde neutral buffer solution and embedded in paraffin; thin sections were cut and stained with hematoxylin-eosin. Arrow and arrow head represent chromatin aggregation and nuclear fragmentation, respectively. Magnification: ×200.