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Bile samples were collected from the gallbladder 12 h after CAR-primed C57BL/6 mice were injected with LPS (A), after D-galN-primed C57BL/6 mice were injected with LPS (B), and from the negative control (C). Each sample was diluted with saline, and then subjected to SDS-PAGE and subsequent western blot analysis. The expression of sCD14 on the nitro cellulose membrane was detected using an anti-mouse CD14 polyclonal antibody as a probe.
