Table 2. Best fit parameters for the biphasic activation-inhibition dose-response curves with DAPT^a.

ng/mL of pSG5-cDNAwtC99 used to transfect HeLa cells
Data from Fig. 2A a :	0	15	25	61	92	Average
Initial Activity, IA	32 ± 3	41 ± 3	51 ± 5	91 ± 6	119 ± 4	n.a. c
[2σ CI] b	[27, 37]	[34, 46]	[43, 59]	[81, 99]	[111, 127]
Maximal Activity, MA	217 ± 110	172 ± 90	205 ± 80	180 ± 145	170 ± 95	189
[2σ CI] b	[470, 133]	[380, 120]	[510, 121]	[430, 160]	[-, -]
Maximal Inhibition, MI	-1 ± 0.1	0.33 ± 0.7	-0.2 ± 0.1	1.2 ± 0.2	1.9 ± 0.6	0.5 ± 0.2
[2σ CI] b	[-1.75, 1.1]	[-1.0, 2.1]	[-1.5, 0.9]	[-1.36, 0.86]	[-2.7, 2.9]
Activation EC50, nM	89 ± 17	71 ± 21	110 ± 34	107 ± 36	178 ± 45	111 ± 41
[2σ CI] b	[50, 240]	[105, 243]	[53, 251]	[22, 160]	[-, -]
Activation Hill coef.	1.04 ± 0.2	1.08 ± 0.2	1.0 ± 0.1	1.13 ± 0.6	0.92 ± 0.3	1.0 ± 0.1
[2σ CI] b	[0.85, 1.35]	[0.9, 1.45]	[0.9, 1.3]	[0.87, 1.28]	[0.61, 1.5]
Inhibition IC50, nM	407 ± 34	588 ± 35	524 ± 71	489 ± 62	661 ± 80	534 ± 53
[2σ CI] b	[479, 724]	[269, 550]	[457, 562]	[150, 602]	[525, 732]
Inhibition Hill’s coef	2 ± 0.3	2.4 ± 0.3	2.2 ± 0.4	2.3 ± 0.5	1.7 ± 0.6	2,1 ± 0.2
[2σ CI] b	[1.4, 2.9]	[1.6, 3.13]	[1.6, 2.9]	[1.6, 3.0]	[-, -]

The units for all activity measurements are: Aβ 1-40 secreted in pM per 10⁶ cells.

^athe best fit values ± standard error were calculated using nonlinear regression and eqn.1.

^btwo sigma confidence intervals as indicated in methods section [34].

^cnot applicable, or the corresponding values could not be resolved in the current experiments.

^dno activation is observed when DNA concentration is higher than 250 ng/ml. Thus starting at 250 ng/ml the best fit values ± standard error were calculated using a nonlinear regression and eqn.2.