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Panel A; Protein G purification. Panel B; Protein A purification. Lane 1 (all panels); Mark 12 standard. FT; flow-through, W; wash, E; elution. Panel C; papain digestion containing Fab and Fc fragments was fractionated on immobilised Protein A column. Flow through (FT) fraction contained the Fab fragment (a doublet of approximately 25 kDa).
