Figure 6. Reactivity of rabbit antibodies to P. alecto IgGH and IgMH in P. alecto serum.
Panel A. P. alecto serum samples (neat and 1∶5) were separated by reducing SDS-PAGE and probed with rabbit antiserum against P. alecto IgGH (lanes 1 and 2), IgMH (lanes 3 and 4) and Fab fragment (lanes 5 and 6). Panel B. 2-DE separation of P. alecto serum sample (silver stain). Panel C and D, 2-DE separation of P. alecto serum sample probed with rabbit antiserum against P. alecto IgMH (panel C) and IgGH (panel D).