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. 2013 Jan 7;8(1):e53951. doi: 10.1371/journal.pone.0053951

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© 2013 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Representative sirius red staining for fibrosis in FVB and Mb hearts. (B) Semiquantitative scores for fibrosis staining performed as described in Methods by a blind observer. Staining of fibrosis in Mb-TAC hearts was significantly higher than that in FVB-TAC hearts. The expression of collagen 1 mRNA (panel C) measured by quantitative RT-PCR was significantly higher in Mb-TAC hearts than in FVB-TAC hearts. Expression of collagen 3 mRNA (panel D) was elevated in TAC hearts but not significantly different in Mb-TAC versus FVB-TAC hearts. #P<0.05, Mb-TAC vs. FVB-TAC and *P<0.05, Sham vs. TAC by 2-way ANOVA, n = 5 for each group.