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. 2013 Jan 7;8(1):e53717. doi: 10.1371/journal.pone.0053717

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© 2013 Charoenthongtrakul et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, C) 293T cells were transfected with 1 μg of either HA-TRAF3, Flag-IKKi, Flag-TBK1 or RNF11-GFP. Co-IPs were conducted using anti-Flag followed by immunoblotting with anti-HA and anti-Flag. Immunoblotting was performed with lysates using anti-Flag, anti-HA, anti-GFP and anti-Actin. (B, D) 293T cells were transfected with IFN-β luciferase reporter (200 ng), pRL-tk (20 ng), and 1 µg of RNF11, TRAF3, IKKi or TBK1. Dual luciferase assays were performed with protein lysates 24 h later. *, p<0.05. Error bars, S.D.