Table 2. Deletion or addition of N-glycan on the glycoprotein modifies LCM virus fitness towards mouse neurons or macrophages.

Mutation	Cell line MOI 0.01		Primary cell MOI 0.01		Primary cell MOI 2
	OBL21a	RAW 264.7	Neurons	BMDM	Neurons	BMDM
F260L	+1.91 ↑	+5.92 ↑	+0.22	+3.85 ↑	+0.58	+2.37 ↑
Deletion of N-glycosylation site
T126A (5)	+0.67	−0.46	+0.02	−0.22	−0.58	−0.67
T234A (7)	+0.96	−1.00	+4.32 ↑	−1.41 ↓	−1.82 ↓	−1.83 ↓
S398A (10)	+0.94	−1.90 ↓	+3.34 ↑	−1.41 ↓	−0.57	−3.32 ↓
T403A (11)	+0.84	−2.69 ↓	+2.32 ↑	−0.9	−2.21 ↓	−4.45 ↓
Addition of N-glycosylation site
G104N (3)	−2.66 ↓	−2.24 ↓	−0.66	−3.81 ↓	−4.78 ↓	−6.69 ↓
E379N/A381T (9)	−2.18 ↓	−1.22 ↓	−1.22 ↓	+0.94	−6.20 ↓	+1.00

Virus production at 24 h post infection for the different rLCMV glycosylation mutants compared to WT in mouse cell line or primary cell and at low (0.01) or high (2) MOI. Virus production calculated as follows: Log 2 (rLCMV mutant titer at 24 h post infection/rLCMV WT titer at 24 h post infection) (N = 4). Decreased virus production −1 (grey ↓) ≤ wt virus production ≥1 increased virus production (bold ↑).