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. Author manuscript; available in PMC: 2013 Jan 8.
Published in final edited form as: Nat Protoc. 2008;3(8):1278–1286. doi: 10.1038/nprot.2008.118

Figure 6.

Figure 6

Imaging of tissue vascularization. Panels show the same graft as in Figure 5. Blood vessels were visualized by an intravenous injection of a 70 kDa Texas Red labeled dextran. GFP (a) and Texas Red (b) were excited with a two-photon laser at 890 nm at minimal necessary laser power required and emission collected onto nondescanned detectors using a dichroic mirror (RSP 560) and emission filters (BP 525/50 and BP 640/20). (c) Overlay of a and b. Objective APO 10 × 0.3 W. Pinhole: 1 airy unit. Zoom factor: 1.7. Image bit depth: 8 bit. Resolution xyz: 1.38 × 1.38 ×5 μm. Scale bar: 100 μm.