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. Author manuscript; available in PMC: 2013 Jan 8.
Published in final edited form as: Nat Protoc. 2008;3(8):1278–1286. doi: 10.1038/nprot.2008.118

Figure 7.

Figure 7

Imaging of cell death. Panels show the same graft as in Figures 5 and 6. β cell death was induced by intravenous injection of alloxan. Apoptotic and dead cells were visualized by intravenous injection of annexin V-APC. (a) Reflection was imaged by exciting with 543 nm and emission measured between 539 and 547 nm at 35% laser power. (b) GFP was excited at 488 nm and emission measured between 495 and 530 nm at 35% laser power. (c) Allophycocyanin (APC) was excited at 633 nm with collection of emission light between 645 and 680 nm at 75% laser power. (d) Overlay of a,b and c. Objective APO 10 × 0.3 W. Pinhole: 1 airy unit. Zoom factor: 1.7. Image bit depth: 8 bit. Resolution xyz: 1.38 × 1.38 × 10 μm. Scale bar: 100 μm.