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. Author manuscript; available in PMC: 2013 Jan 8.
Published in final edited form as: Nat Protoc. 2008;3(8):1278–1286. doi: 10.1038/nprot.2008.118

Figure 8.

Figure 8

Loading of cells within the anterior chamber of the eye with calcium indicators. For loading, the anterior chamber was perfused with Fluo-4 and Fura-Red. Fluo-4 (a) and Fura-Red (b) were excited with 488 nm at 25% laser power and emission measured for Fluo-4 between 495 and 535 nm and for Fura-Red between 600 and 700 nm. Reflection was imaged by exciting with 543 nm and emission measured between 539 and 547 nm at 15% laser power. (c) Overlay of a and b. Objective APO 20 × 0.5 W. Pinhole: 1 airy unit. Zoom factor: 1.7. Image bit depth: 12 bit. Resolution xyz: 0.7 × 0.7 × 3.3 μm. Scale bar: 100 μm.