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Quenching of the intrinsic fluorescence of iNOS by 22.
The [22] was varied from 0–50 RM. The experimental conditions were as follows: excitation = 280 nm; emission = 330 nm; [TrisHCl] = 50 mM; NaCl = 100 mM, pH = 7.5 at 25°C. The fitted line passing through the data is from Eqn. 1 as described in the Experimental Methods. Other reaction conditions are also described.
