Skip to main content
. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: Vaccine. 2012 Mar 23;30(23):3435–3444. doi: 10.1016/j.vaccine.2012.03.023

Fig. 1.

Fig. 1

Sortase-conjugation generates the PDGA-D4 vaccine. (A) Sortase links the amino group of a synthetic poly-γ-D-glutamate (NH2-Gly5-D-γ-Glu10) to the LPETG sorting signal engineered at the C-terminal end of the protective antigen D4 domain. (B) Sortase-conjugation of PDGA to D4 results in a mobility shift of PDGA-D4 on 15% Coomassie-stained SDS-PAGE. (C) Using a prime-two-booster schedule, guinea pigs (n=7) were immunized with either D4 or PDGA-D4 adsorbed to aluminum hydroxide and serum IgG analyzed for immune reactivity to either D4 or PDGA antigen. Data are representative of two independent determinations.