Table 4.
Exact mass measurements for pQIRYHQCYFNPISCF (with disulfide bridge) from the analysis of freshly dissected and desheathed commissural ganglia (CoG) tissue samples using MALDI-FTMS
| Peptide Identity | pQIRYHQCYFNPISCF (with disulfide bridge) | ||
|---|---|---|---|
| Ion Identity | [M+H]+ | ||
| Calculated m/z | m/z 1899.83052a | ||
| Infraorder | Species | Measured m/zb | Error (ppm)c |
| Penaeidae | Farfantepenaeus duorarum | ND | |
| Litopenaeus vannamei | ND | ||
| Caridea | Crangon septemspinosa | ND | |
| Pandalus danae | ND | ||
| Pandalus platyceros | ND | ||
| Stenopodidea | Stenopus hispidus | 1899.82820 | -1.2 |
| Astacidea | Cherax quadricarinatus | 1899.83900 | 4.5 |
| Homarus americanus | 1899.83210 | 0.8 | |
| Homarus gammarus | 1899.83831 | 4.1 | |
| Nephrops norvegicus | 1899.84194 | 6.0 | |
| Pacifastacus leniusculus | 1899.83408 | 1.9 | |
| Procambarus clarkii | 1899.83425 | 2.0 | |
| Thalassinidea | Callianassa californiensis | 1899.83311 | 1.4 |
| Achelata | Panulirus interruptus | ND | |
| Panulirus versicolor | 1899.82738 | -1.6 | |
| Scyllarides latus | 1899.82613 | -2.3 | |
| Anomura | Clibanarius vittatus | 1899.83171 | 0.6 |
| Lithodes maja | 1899.84010 | 5.0 | |
| Pachycheles rudis | ND | ||
| Pagurus acadianus | 1899.83036 | -0.1 | |
| Pagurus granosimanus | ND | ||
| Pagurus pollicaris | 1899.83572 | 2.7 | |
| Petrolisthes cinctipes | 1899.83344 | 1.5 | |
| Petrolisthes eriomerus | ND | ||
| Brachyura | Cancer antennarius | 1899.83800 | 4.0 |
| Cancer borealis | 1899.83252 | 1.0 | |
| Cancer gracilis | 1899.83126 | 0.4 | |
| Cancer irroratus | 1899.83635 | 3.1 | |
| Cancer magister | 1899.83757 | 3.7 | |
| Cancer productus | 1899.83467 | 2.2 | |
| Carcinus maenas | 1899.83064 | 0.1 | |
| Hemigrapsus nudus | 1899.83408 | 1.9 | |
| Lophopanopeus bellus | 1899.83255 | 1.1 | |
| Ovalipes ocellatus | 1899.84186 | 6.0 | |
| Pugettia gracilis | 1899.83685 | 3.3 | |
| Pugettia producta | 1899.83842 | 4.2 | |
| Scyra acutifrons | 1899.82610 | -2.3 |
a
Monoisotopic m/z;
b
Average of calibrated mass measurements. Spectra were calibrated using either poly(propylene glycol), NFDEIDRSGFGFA (Anomura and Brachyura) and APSGFLGMRamide (m/z 934.4927), pQDLDHVFLRFamide (m/z 1271.6531 and m/z 817.4832), and GYRKPPFNGSIFamide (m/z 1381.77375) or VYRKPPFNGSIFamide (m/z 1423.7845). ND = not detected;
c
Mass measurement error in ppm