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. Author manuscript; available in PMC: 2013 Jan 8.
Published in final edited form as: Sci Signal. 2008 Sep 2;1(35):ra1. doi: 10.1126/scisignal.1159945

Fig. 7.

Fig. 7

Knockout of Casr in chondrocytes blocks the expression of IGF-1 and IGF-1R and delays cell differentiation. (A) Immunohistochemical staining of IGF-1 and IGF-1R, depicted by brown DAB staining, in the proximal tibial growth plates from E18 to E19 Tam-Cart-KO and control mice. (B) In vitro knockout of IGF-1R was performed by infecting floxed IGF-1R-containing GPCs with Ad-Cre (Cre) or, as a control, Ad-Cont (Cont) viruses (16 PFU/cell). Expression of IGF-1R and Col(X) mRNAs was assessed by qPCR 72 hours postinfection. (C) Western blots were incubated with antisera against the β subunit of IGF-1R to confirm the knockdown of IGF-1R in GPCs infected with Ad-Cre (Cre) compared with that in Ad-Cont (Cont) viruses. (D) AR staining was performed and quantified by absorbance to assess mineralization in cultures infected with Ad-Cre (Cre) or Ad-Cont (Cont) viruses and grown in media containing 2.0 mM Ca2+ for 14 days (**P < 0.001; n = 3).