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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Traffic. 2012 Nov 12;14(2):194–204. doi: 10.1111/tra.12020

Figure 5. Mitochondria-targeted COG6 specifically relocates COG8 and CFP-GS27.

Figure 5

(A) A schematic diagram of the mitochondrial targeting protein constructs. HeLa cells were co-transfected with mChActA and CFP-GS27(B), mChActA and CFP-STX6 (C), COG6-mChActA and YFP-COG3 (D), COG6-mChActA and COG8-GFP (E), COG6-mChActA and CFP-STX6 (F), COG6-mChActA and CFP-GS27 (G), COG6Δ76-150-mChActA and CFP-GS27 (H), COG6Δ76-150-mChActA and GFP-Rab6Q72L (I), and then fixed and analyzed 24 h after transfection using confocal microscopy. Cells were stained with antibodies to GM130 (B-D, F- I) to identify Golgi, or mitochondria resident protein Ox Phos ComplexV (E). Mitochondria are not clustered in cells expressing mChActA (A, B), and are clustered in cells expressing COG6-mChActA. Line plots for overlap between red, green and blue channels are shown. Scale bar, 10 μm.