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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Eur J Neurosci. 2012 Nov 8;37(1):118–129. doi: 10.1111/ejn.12027

Figure 1. NAAG is present at the lizard NMJ.

Figure 1

A. NAAG (green) is present at the lizard NMJ junction, imaged using differential interference contrast (DIC), and is not in the muscle cells (N=11, n=165). B. Separate planes from a 3D Z-stack of a terminal stained for NAAG (green) and nicotinic ACh receptors using α-bungarotoxin (red). The Z-stack is presented in 1μm increments vertically through the field. Zoom of B shows that NAAG is consistently encapsulated within the nerve terminal. C. Two neighboring synapses show variability in presence of NAAG (green) within the nerve terminal (red, α-bungarotoxin). 73% of synapses had NAAG present at time of fixation (see figure 2). The image shown is a maximum projection of 16 images collected at 0.5 μm increments vertically through the field containing the NMJ. D. NAAG (green) does not always fill the whole terminal (red, α-bungarotoxin). The middle set of boutons still retains its NAAG, while the upper and lower sets of boutons are devoid of NAAG immunofluorescence. The image shown is a maximum projection of 21 images collected at 0.27 μm increments vertically through the field containing the NMJ. Calibration bars, 10 μm.