A. Perisynaptic Schwann Cell (PSC) unablated preparation shows GCPII (green) stain at the nerve terminal (DAPI in blue indicating PSC nuclei). (N=4, n=100). B. Ablated preparation lacks GCPII (green) where ablated PSCs are marked with EthD-1 (red) and DAPI (blue) (N=2, n=50). For both panels A and B, an image collected using DIC optics is superimposed onto fluorescent images collected using DAPI, FITC and TRITC filter cubes. Calibration bars, 10 μm.