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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Eur J Neurosci. 2012 Nov 8;37(1):118–129. doi: 10.1111/ejn.12027

Figure 3. Immunohistochemical staining of NAAG hydrolase enzyme, GCPII.

Figure 3

A. Perisynaptic Schwann Cell (PSC) unablated preparation shows GCPII (green) stain at the nerve terminal (DAPI in blue indicating PSC nuclei). (N=4, n=100). B. Ablated preparation lacks GCPII (green) where ablated PSCs are marked with EthD-1 (red) and DAPI (blue) (N=2, n=50). For both panels A and B, an image collected using DIC optics is superimposed onto fluorescent images collected using DAPI, FITC and TRITC filter cubes. Calibration bars, 10 μm.