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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Eur J Neurosci. 2012 Oct 29;37(1):1–9. doi: 10.1111/ejn.12037

Figure 4. Three-dimensional reconstruction of confocal images.

Figure 4

DiI labeled medium spiny neurons in NAc were imaged and DiI was excited using the Helium/Neon 543 nm laser line. Each neuron was scanned at 0.15μm intervals along the z-axis (maximum 90 planes, depending on the depth of whole neuron and objective WD), and the morphology of the dendritic tree was reconstructed in 3-D for analysis with IMARIS software.