Table A1.

Characteristics of five microsatellite loci from 18 collections of Siganus spinus (>60 mm FL) from the western Pacific. Given are: Site code and sample size (N), number of alleles (A), observed heterozygosity (H_O), expected heterozygosity (H_E), and inbreeding coefficient (F_IS)¹

Sample code (N)		Sfus-113	Sfus-56	Sfus-8	Sfus-9	Sfus-98
PID	A	13	2	19	2	2
88	HO	0.824	0.011	0.852	0.264	0.138
	HE	0.851	0.011	0.919	0.229	0.185
	FIS	0.038	0.000	0.080	–0.147	0.262
PAB	A	15	2	18	2	2
50	HO	0.804	0.040	0.786	0.146	0.163
	HE	0.858	0.039	0.918	0.170	0.215
	FIS	0.074	–0.010	0.156	0.152	0.250
PAK	A	12	2	15	2	2
40	HO	0.917	0.050	0.750	0.179	0.175
	HE	0.845	0.049	0.890	0.242	0.160
	FIS	–0.070	–0.013	0.171	0.271	–0.083
YAW	A	9	2	13	2	2
26	HO	0.760	0.154	0.692	0.192	0.269
	HE	0.793	0.142	0.894	0.233	0.286
	FIS	0.062	–0.064	0.244	0.194	0.079
PNK	A	8	2	9	2	2
9	HO	0.875	0.111	0.667	0.222	0.111
	HE	0.820	0.105	0.852	0.198	0.401
	FIS	0.000	0.000	0.273	–0.067	0.750
GUC	A	9	2	12	1	3
26	HO	0.846	0.038	0.583	0.000	0.269
	HE	0.798	0.038	0.852	0.000	0.240
	FIS	–0.041	0.000	0.334	NA	–0.101
GUG	A	8	2	12	1	2
39	HO	0.744	0.026	0.447	0.000	0.205
	HE	0.730	0.025	0.896	0.000	0.184
	FIS	–0.005	0.000	0.511	NA	–0.101
GUI	A	12	2	15	2	3
67	HO	0.800	0.015	0.621	0.015	0.258
	HE	0.772	0.015	0.889	0.015	0.275
	FIS	–0.028	0.000	0.310	0.000	0.072
GUP	A	11	2	12	1	2
28	HO	0.821	0.107	0.583	0.000	0.250
	HE	0.783	0.101	0.856	0.000	0.219
	FIS	–0.032	–0.039	0.337	NA	–0.125
GUT	A	12	2	13	2	3
87	HO	0.720	0.048	0.579	0.011	0.184
	HE	0.769	0.046	0.895	0.011	0.187
	FIS	0.070	–0.018	0.359	0.000	0.020
SAC	A	8	2	11	1	3
18	HO	0.750	0.111	0.647	0.000	0.333
	HE	0.768	0.105	0.808	0.000	0.285
	FIS	0.055	–0.030	0.228	NA	–0.140
SAL	A	10	2	13	1	3
56	HO	0.745	0.055	0.648	0.000	0.268
	HE	0.753	0.053	0.891	0.000	0.236
	FIS	0.019	–0.019	0.281	NA	–0.126
SAW	A	9	2	10	2	2
19	HO	0.684	0.105	0.500	0.053	0.368
	HE	0.715	0.100	0.859	0.051	0.361
	FIS	0.070	–0.029	0.444	0.000	0.008
CHP	A	12	1	16	2	2
92	HO	0.800	0.000	0.525	0.258	0.228
	HE	0.789	0.000	0.910	0.273	0.202
	FIS	–0.009	NA	0.428	0.058	–0.124
CHX	A	12	1	12	2	2
51	HO	0.780	0.000	0.405	0.362	0.216
	HE	0.799	0.000	0.876	0.347	0.192
	FIS	0.034	NA	0.547	–0.032	–0.111
POS	A	12	1	15	2	2
114	HO	0.738	0.000	0.524	0.167	0.257
	HE	0.786	0.000	0.902	0.181	0.237
	FIS	0.066	NA	0.423	0.086	–0.080
PON	A	13	1	14	2	2
108	HO	0.800	0.000	0.522	0.279	0.241
	HE	0.799	0.000	0.898	0.254	0.265
	FIS	0.004	NA	0.424	–0.095	0.097
MAA	A	10	1	12	2	2
53	HO	0.792	0.000	0.413	0.113	0.377
	HE	0.816	0.000	0.878	0.107	0.306
	FIS	0.038	NA	0.537	–0.051	–0.224

¹Numbers in boldface type indicate significant departure from Hardy–Weinberg equilibrium at the 0.05 level after Bonferroni correction.